Application of immobilized enzymes for the paper-splitting process
| Hlavní autori: | , , , , |
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| Médium: | Kapitola |
| Jazyk: | angličtina slovenčina |
| ISBN: | ISBN 1-84407-253-3 |
| On-line prístup: | http://www.viks.sk/chk/14tmh_34.doc |
| Abstrakt: | SUMMARIES. The study deals with the improvement of the last step of the paper-splitting process: the removal of the facing supports formerly adhered to the original document from both sides with gelatin solution. Experiments were undertaken to test whether it is possible to use immobilized proteases instead of enzyme solutions for the support removal. Corolase, thermolysin, trypsin and a new protease were immobilized to new synthetic support material and activated at elevated temperatures for digesting the gelatin. The new technique was assessed by a conservator and biochemical analysis. The study was part of a joint research project of the Institute for Preservation of Archival and Library Material, Ludwigsburg, and the Institute for Physiological Chemistry of Tübingen University. It was supported generously by the German Research Association (Deutsche Forschungsgemeinschaft, DFG). CONCLUSIONS. Immobilized proteases are able to replace the commercial enzyme solutions used up to now during the paper-splitting process. Trypsin fixed to reusable polyester non-woven material liquefies the gelatin adhesive between support and original document after the splitting process and the insertion of the core. The immobilized enzyme becomes activated by elevated temperature and humidity just at the time chosen for support removal. It does not obstruct the other steps of the restoration treatment, even though it is in direct contact with the document. This technique avoids the disadvantages of immersing the documents into baths with crude protease solutions as required traditionally. Further work must be performed to adapt the method for routine application. In particular, the procedure of immobilizing enzymes to many standard sized sheets of non-woven must be scaled up. |
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| ISBN: | ISBN 1-84407-253-3 |
