| Summary: | SUMMARIES. The subject matter of the microbiological studies was a manuscript of the New Testament dating back to the turn of the 9th century, bought by the National Library in 1986. The manuscript was in a very bad condition, the parchment leafs were in some places loosened and discoloured, covered with bloom, spots and with clearly visible defects. The purpose of the microbiological studies was to find an explanation for the decay of parchment leafs and to select the most effective disinfecting agent. No traces of fungi and actinomycetes were found in the tested leaf samples. However tests indicated the presence of anaerobic and aerobic bacteria in them. Preliminary identification of obtained bacterial strains indicated the presence of Gram-positive resting spores: the aerobic Bacillus and anaerobic Clostridium. In the next stage of studies, the sensitivity of isolated strains of bacteria to selected disinfecting agents was tested: Aseptina P, Lichenicida and p-chloro-m-krezol. Based on study results, it was found that p-chloro-m-krezol was the most effective disinfecting agent for the tested bacteria strains and therefore, the application of this agent was justified.
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