Paper Scientific Developments: The characterization of enzymes for use in paper conservation

Bibliographic Details
Main Authors: Grattan, David (Author), Hilaire, Johanne St (Author), Burgess, Helen D. (Author), McCawley, Clifford (Author)
Format: Article
Language:English
Slovak
ISBN:ISBN 0-408-01466-0
Online Access:http://www.viks.sk/chk/conservation_book_15_24.doc

MARC

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100 1 |a Grattan, David  |4 aut  |9 770872 
245 1 0 |a Paper  |b Scientific Developments: The characterization of enzymes for use in paper conservation 
520 3 |a Conclusions. Using the characterization information shown here the conservator can choose the most favourable conditions for the artifact in question. It is quite clear that enzymes are useful reagents even at room temperatures. Perhaps the second most useful conclusion is the benefit of using wetting agents to speed the action, particularly with sized papers. 
520 3 |a Summaries. Enzymes may become important tools in paper conservation because they act with specificity and rapidity. Proteases act only upon proteins and amylases only on starches. Their use depends on knowledge of the activity of the enzyme and of the composition of the artifact. Performance depends on pH, temperature, the ability to penetrate and the concentration of the enzyme. Commercially available enzymes vary in their composition; they are often mixtures of several enzymes and their activity and specificity may not be precisely known. Small increases in temperature can cause huge increases in activity at the expense of reducing the period during which the enzyme is active; however, if the temperature is high enough, the enzyme denatures and the activity falls to zero. An enzyme for use in conservation ought to be a well characterized reagent, so that treatment conditions can be controlled exactly. In many instances it is important to minimize the soaking period of an object; furthermore, enzymes tend to be expensive; thus it is important to optimize the treatment conditions. This paper describes the characteristics of some proteases and amylases. The effect of temperature, pH, concentration, the shelf life of enzyme solutions, the effectiveness of enzymes as releasing agents for adhesives and the permeability of enzymes through various kinds of paper were all investigated. 
700 1 |a Hilaire, Johanne St.  |4 aut  |9 1071718 
700 1 |a Burgess, Helen D.  |4 aut  |9 1071719 
700 1 |a McCawley, Clifford  |4 aut  |9 1071720 
773 |t Conservation of Library and Archive Materials and the Graphic Arts  |b first published  |d Kent: Butterworths 1987, s. 15-24  |z ISBN 0-408-01466-0 
856 4 |u http://www.viks.sk/chk/conservation_book_15_24.doc 
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