Identification of Living Fungi on Archives and Library Materials
| Main Authors: | , , |
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| Format: | Article |
| Language: | English German |
| ISBN: | ISBN 3-8062-1925-7 |
| Online Access: | http://www.viks.sk/chk/schimmel6.doc |
| Abstract: | SUMMARIES. Various rapid test methods based on bioluminescence and the determination of ATP (adenosine triphosphate) are described. The techniques for measuring ATP, ADP, AMP and EC (energy charge) to determine living and dead fungi on materials in archives and libraries are applied for the first time. Results of the EC measurements for different species after treatment with sterilising agents and water vapour are presented. CONCLUSION. The method for measuring ATP, ADP, AMP and EC in order to determine living and dead fungi on archives and library materials is applied in this study. Identification of living and dead microorganisms can be determined within a period of up to 1 hour - it ranks this method as very fast and at the same time very sensitive. Detection limit has been determined within the range of 50-100 spores per cuvette in luminometer. A minimum of 5 repeats for one assay is recommended. Experiments with spores disinfected with ethylene oxide confirm our previous results that ATP is not totally degraded in cells treated with this gas. We suppose that ethylene oxide inhibits spores activity but possibly pro-:ects the ATP present, preventing it from being degraded. Ethylene oxide has certainly an effect on enzymes (ATPases) responsible for the degradation of the ATP at the death of the cells. For this reason, it is necessary to calculate the energy charge values, which are more representative of the viability of cells. The average energy charge values measured on different living moulds are within the range of 0,81-1,00. The EC values for moulds treated by vapour - i. e. dead moulds -are within the range of 0,45-0,58. The EC values for ethylene oxide treated moulds are below 0,67. These results confirm that the methods is suitable and appropriate for given purposes as it can distinguish between living and dead moulds clearly and without problem. The method is also applicable on a mixture of different moulds. In such case it is sufficient if only one living mould occurs among the others in order to get EC value over 0,8. |
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| ISBN: | ISBN 3-8062-1925-7 |
